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live deadtm fixable dead cell stain kit  (Thermo Fisher)


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    Thermo Fisher live deadtm fixable dead cell stain kit
    Live Deadtm Fixable Dead Cell Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+deadtm+fixable+dead+cell+stain+kit/Phosphate/pmc12529554-45-6-12
    Average 99 stars, based on 1 article reviews
    live deadtm fixable dead cell stain kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture
    Article Snippet: .. After centrifugation and washing the cell pellet, cells were incubated with LIVE/DEADTM Fixable Dead Cell Stain Kit (invitrogen) for 15 min. After that period, cells were washed with PBS, resuspended in PBS for a final volume of 300 μL/FACS tube and run in flow cytometer for cell viability data acquisition. .. Total protein extraction and Immunoblotting hiPSCs were collected and lysed using ice-cold lysis buffer (10 mM Tris-HCl, pH 7.6, 5 mM MgCl2, 1.5 mM potassium acetate, 1% Nonidet P-40, 2 mM DTT) and 1X Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Inc.) for 30 min at 4 °C.

    Incubation:

    Article Title: Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture
    Article Snippet: .. After centrifugation and washing the cell pellet, cells were incubated with LIVE/DEADTM Fixable Dead Cell Stain Kit (invitrogen) for 15 min. After that period, cells were washed with PBS, resuspended in PBS for a final volume of 300 μL/FACS tube and run in flow cytometer for cell viability data acquisition. .. Total protein extraction and Immunoblotting hiPSCs were collected and lysed using ice-cold lysis buffer (10 mM Tris-HCl, pH 7.6, 5 mM MgCl2, 1.5 mM potassium acetate, 1% Nonidet P-40, 2 mM DTT) and 1X Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Inc.) for 30 min at 4 °C.

    Staining:

    Article Title: Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture
    Article Snippet: .. After centrifugation and washing the cell pellet, cells were incubated with LIVE/DEADTM Fixable Dead Cell Stain Kit (invitrogen) for 15 min. After that period, cells were washed with PBS, resuspended in PBS for a final volume of 300 μL/FACS tube and run in flow cytometer for cell viability data acquisition. .. Total protein extraction and Immunoblotting hiPSCs were collected and lysed using ice-cold lysis buffer (10 mM Tris-HCl, pH 7.6, 5 mM MgCl2, 1.5 mM potassium acetate, 1% Nonidet P-40, 2 mM DTT) and 1X Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Inc.) for 30 min at 4 °C.

    Article Title: Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity
    Article Snippet: .. After 4h seeding and 1 and 7 days of culture, dead cells were stained for 20 minutes prior to fixation using the LIVE/DEADTM Fixable Dead Cell Stain Kit (Thermo Fisher Scientific) at a concentration of 0.5 μl stain in 500 μl Hank’s Balanced Salt Solution per scaffold. ..

    Article Title: Extended SARS-CoV-2 RBD booster vaccination induces humoral and cellular immune tolerance in mice
    Article Snippet: .. LIVE/DEADTM Fixable Dead Cell Stain Kit , Invitrogen , Cat#L34976. .. Mouse and Rat cytokine Assays kit , Bio-RAD , Cat#10014905.

    Article Title: A long-term culture model for investigating senescence-associated dysregulation in macrophages
    Article Snippet: .. Cell viability was evaluated using the LIVE/DEADTM Fixable Dead Cell Stain Kit (Thermo Fisher Scientific, US) at a 1:1000 dilution in phosphate-buffered saline (PBS) following the manufacturer’s protocol. .. After viability staining, surface marker staining was performed at 4 °C for 30 minutes in the presence of BD HorizonTM Brilliant Stain Buffer (BD Biosciences) to minimize fluorochrome spillover and spectral overlap.

    Article Title: Extended SARS-CoV-2 RBD booster vaccination induces humoral and cellular immune tolerance in mice
    Article Snippet: .. LIVE/DEADTM Fixable Dead Cell Stain Kit (Invitrogen: #L34976) was used for viability dye staining. .. For surface staining, splenocytes were stained with the following antibodies: APC anti-mouse CD19 (Clone: 1D3/CD19, Biolegend), PE anti-mouse CD138 (Syndecan-1) (Clone: 281-2, Biolegend), FITC anti-mouse/rat/human CD27 (Clone: LG.3A10, Biolegend), PE anti-mouse CD279 (PD-1) (Clone: 29F.1A1, Biolegend), PE/Cyanine7 anti-mouse CD4 (Clone: GK1.5, Biolegend), and APC/Cyanine7 anti-mouse CD185 (CXCR5) (Clone: L138D7, Biolegend) for Tfh cell analysis; with FITC anti-mouse/human GL7 Antigen (Clone: GL7, Biolegend), PerCP/Cyanine5.5 anti-mouse CD95 (Fas), (Clone: SA367H8, Biolegend), and Alexa Fluor® 647 anti-mouse/human CD45R/B220(Clone: RA3-6B2, BD PharmingenTM) mAb for GC B cell analysis.

    Flow Cytometry:

    Article Title: Transcriptomic analysis of 3D Cardiac Differentiation of Human Induced Pluripotent Stem Cells Reveals Faster Cardiomyocyte Maturation Compared to 2D Culture
    Article Snippet: .. After centrifugation and washing the cell pellet, cells were incubated with LIVE/DEADTM Fixable Dead Cell Stain Kit (invitrogen) for 15 min. After that period, cells were washed with PBS, resuspended in PBS for a final volume of 300 μL/FACS tube and run in flow cytometer for cell viability data acquisition. .. Total protein extraction and Immunoblotting hiPSCs were collected and lysed using ice-cold lysis buffer (10 mM Tris-HCl, pH 7.6, 5 mM MgCl2, 1.5 mM potassium acetate, 1% Nonidet P-40, 2 mM DTT) and 1X Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, Inc.) for 30 min at 4 °C.

    Concentration Assay:

    Article Title: Improving cell distribution on 3D additive manufactured scaffolds through engineered seeding media density and viscosity
    Article Snippet: .. After 4h seeding and 1 and 7 days of culture, dead cells were stained for 20 minutes prior to fixation using the LIVE/DEADTM Fixable Dead Cell Stain Kit (Thermo Fisher Scientific) at a concentration of 0.5 μl stain in 500 μl Hank’s Balanced Salt Solution per scaffold. ..

    Saline:

    Article Title: A long-term culture model for investigating senescence-associated dysregulation in macrophages
    Article Snippet: .. Cell viability was evaluated using the LIVE/DEADTM Fixable Dead Cell Stain Kit (Thermo Fisher Scientific, US) at a 1:1000 dilution in phosphate-buffered saline (PBS) following the manufacturer’s protocol. .. After viability staining, surface marker staining was performed at 4 °C for 30 minutes in the presence of BD HorizonTM Brilliant Stain Buffer (BD Biosciences) to minimize fluorochrome spillover and spectral overlap.



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